Skip to main content
[Preprint]. 2024 Aug 28:2023.10.20.562785. Originally published 2023 Oct 23. [Version 2] doi: 10.1101/2023.10.20.562785

Figure 1. High tonic LC activity during hot plate test is not necessary for stress-induced antinociception.

Figure 1.

(A) Cartoon illustrating the viral strategy for stGtACR2 expression in LC. (B) Representative whole-cell recording demonstrating stGtACR2-mediated inhibition of spontaneous firing rate in an LC neuron. (C) Quantification of stGtACR2-mediated inhibition of spontaneous LC activity. (D) Light power intensity response curve showing 2 mW of 470 nm light illumination is sufficient to suppress the spontaneous activity. (E) Representative whole-cell traces demonstrating efficacy of stGtACR2-mediated inhibition of LC neurons against current injections. (F) Quantification of stGtACR2-mediated inhibition against current injections. (G) Cartoon and fluorescent image of the viral strategy for stGtACR2 expression in LC and bilateral fiber optics implanted above the LC. (H) Inhibition of LC neurons following restraint stress does not alter jump latency on a hot plate, but inhibition of LC neurons in stress-naïve mice is antinociceptive. Two-way ANOVA followed by Tukey’s posthoc test, F = 3.901 (stress); 28.13 (photo-Stim.); 5.006 (interaction), ** p<0.01. Data represented as mean ± SEM.