Figure 5. Distinct coding properties and population neural trajectories of MOs-c PN types during RGD.

(A) Projection-defined intratelencephalic (IT) and extratelencephalic (PT, CT) neuron types. The same color code is used in subsequent panels.

(B) Activity tuning by target location from example IT^cux1, IT^P1xnD1,PT^Fezf2 and CT^T1e4 neurons. Vertical dashed lines: advance endpoint.

(C) Spiking activity of all identified neurons aligned to advance endpoint (vertical dashed line). IT^Cux1, IT^P1xnD1, PT^Fezf2 and CT^T1e4 neurons are arranged from top to bottom. Each row represents activity across five target locations. For each target location, z-score normalized activity from 1 second before to 1 second after the advance endpoint is shown. Within a neuron type, individual neurons are sorted by their cluster membership (see Figure 4C).

(D) Average spiking activity of all identified neurons aligned to advance endpoint (vertical dashed lines). Note the longer peak latency and more sustained firing in CT^T1e4 than PT^Fezf2. Last row shows merged activity traces of all types.

(E) Proportion of identified PNs that were assigned to each activity cluster identified in Figure 4C. Note the different membership assignment of PT^Fezf2 and CT^T1e4 neurons. Circle size indicates the proportion of neurons. n.s., not significantly modulated.

(F) Different PN types show highly distinct population neural trajectories. Whereas PT^Fezf2 population dynamics display target location modulated trajectories that resemble those of the whole MOs-c population (Figure 4G), CT^T1e4 trajectories show less target dependency, and IT^Cux1 and ITP^1xnD1 show no clear population trajectory. Median lift, advance endpoint, and first hand lick time points are indicated by triangles, circles, and crosses, respectively.