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[Preprint]. 2024 Nov 1:2023.10.28.564543. Originally published 2023 Oct 28. [Version 3] doi: 10.1101/2023.10.28.564543

Extended Data Figure 3: Extended data on human organoid generation and characterization.

Extended Data Figure 3:

(a,h) Representative images of hiPSC aggregation and immature spheroids post dislodging. (b,i) Normalized organoid perimeters over time (compared to averaged control), hCO (n = 6 donors | 2 batches | 72–161 organoids) and hSO (n = 6 donors | 2 batches | 46–134 organoids). (c, j) RT-qPCR results from 4-month organoids of genes for pluripotency, neuronal, and cell-type specific markers. (d-e, k-l) RT-qPCR results of NRXN1 WT and MT expression hCO (n = 6 donors | 1 representative batch | 12 samples) and hSO (n = 6 donors | 1 representative batch | 12 samples). Statistical tests used were 1-way ANOVAs with Dunnett’s test. (f, m) relative proportions of cell clusters across individual donors, (hCO = 47,460 cells) and (hSO = 35,563 cells). (g, n) Comprehensive gene expression panel across sub-clusters of hCO and hSO samples across neuronal, cortical, subpallial and astroglia markers. Data corresponds to Fig 2.