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[Preprint]. 2023 Nov 1:2023.10.27.564474. [Version 1] doi: 10.1101/2023.10.27.564474

Figure 1. Recombination of the Osmrfl Locus in Tissues from Osmrfl/fl Mx1-Cre Mice.

Figure 1.

Agarose gel electrophoresis (2% agarose) of PCR-amplified products. In all gels a 100bp ladder was used for size estimation located in lane 0. (A) Genotyping PCR for Osmr<tm1.1Nat>. Lanes 1–3 use genomic DNA samples from Mx1-Cre control mice, lanes 4–6 use genomic DNA samples from Osmrfl/fl Mx1-Cre mice, lane 7 uses genomic DNA from a wild-type C57BL/6J mouse and lane 8 uses a positive control Osmrfl/fl from a mouse confirmed by The Jackson Laboratory’s in-house genotyping service. (B) Genotyping PCR for Mx1-Cre. Lane 1 shows a positive control, lane 2 shows wild-type C57BL/6J, lanes 3–5 show Mx1-Cre control mice and lanes 6–8 show Osmrfl/fl Mx1-Cre mice. (C) Recombination PCR assay results in control, bone marrow, lung, liver, and kidney tissues. From left: Lanes 1–2 show wild-type C57BL/6J control mice. In the remaining images, samples are ordered as lanes 1–3 being Mx1-Cre control samples and lanes 4–6 being Osmrfl/fl Mx1-Cre samples.