Thermal characteristics of the source environment for each population listed in order of decreasing latitude (i.e., north to south). Values represent averages from 2000 – 2020, calculated from PRISM climate data at a 1 km buffer around the sampled tree hole. Seasonal temperature variation is defined as the difference between the mean warmest month temperature and the mean coolest month temperature. Warm-season maximum is defined as the mean daily maxima in the Spring and Summer. The # days > 35 or 31.6°C refer to the average number of days where the maximum temperatures exceeded the stated threshold, either across the year, or when considering only non-dormant periods (January – July) or adult activity periods (March – July). See Supplemental Figure S18 for correlations between temperatures variables and Supplemental Figure S19 for comparisons between the PRISM and iButton temperature estimates for the ‘SB’ and ‘POW’ populations.