Source	Number of targets (genes)
bulk RNA-seq DEG, 50 top up/downregulated	100
T-cell related genes	51
KEGG TCR signaling pathway	35
RNA-binding proteins	27
Replogle*: unfolded protein response	51
Replogle: proteasome	41
Replogle: NFkB	20
Replogle: cell cycle	29
Replogle: targets of nonsense-mediated decay	5
Controls (cell cycle, mitochondrial, Cas9)	15
Total number of targets	374

^*“Replogle” prefix means that this category of targets was derived from the published genome-scale Perturb-seq dataset⁵⁰.