Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-RFP primary antibody | Rockland | Cat# 600-401-379 |
| anti-rabbit 550 secondary antibody | Thermo Scientific | Cat# PISA510039 |
| TotalSeq™-A 0451 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody | BioLegend | Cat# 682205 |
| TotalSeq™-A 0452 anti-Nuclear Pore Complex Proteins | BioLegend | Cat# 682207 |
| Hashtag 2 Antibody | ||
| TotalSeq™-A 0453 anti-Nuclear Pore Complex Proteins Hashtag 3 Antibody | BioLegend | Cat# 682209 |
| Bacterial and virus strains | ||
| AAV8-hSyn-FLEX-TVA-P2A-eGFP-2A-oG | Salk Institute for Biological Studies | Custom Prep. by GT3 Core |
| EnvA-rabies-deltaG-H2b-mCherry | Salk Institute for Biological Studies | Custom Prep. by GT3 Core |
| AAVDJ-DIO-H2b-mCherry | Tao et al., 2021 | NA |
| AAV8-hSyn-DIO-mCherry | Addgene | 50459 |
| AAV1-hSyn-Flex-GCaMP 6s | Addgene | 100845 |
| pAAV-EF1alpha-dFlox-hChR2-mCherry | Addgene | 20297 |
| pAAV-EF1alpha-dFlox-hChR2-eYFP | Addgene | 20298 |
| AAV-DJ-CM V-DIO-EGFP-2A-TeNT | gifted from Richard Palmiter | |
| Chemicals, peptides, and recombinant proteins | ||
| Leptin | R & D Systems | 498-OB |
| Liraglutide | Selleckchem | S8256 |
| KCl | Sigma Aldrich | 7447-40-7 |
| Picrotoxin | Abcam | ab120315 |
| NBQX | Abcam | ab120046 |
| DL-AP5 | Abcam | ab120004 |
| TSA Plus Cy5 | Akoya Biosciences | Cat# TS-000203 |
| Critical commercial assays | ||
| RNAscope Multiplex Fluorescent Reagent Kit v2 | Advanced Cell Diagnostics | Cat# 323110 |
| RNAscope H202 and Protease Reagents | Advanced Cell Diagnostics | Cat# 322381 |
| Chromium Next GEM Chip M Single Cell Kit | 10X Genomics | Cat# 1000371 |
| Deposited data | ||
| Raw and processed single-nucli RNA-seq data files | GEO | |
| Clustered single nuclei RNA-seq data | Broad Single Cell Portal | |
| Experimental models: Organisms/strains | ||
| Mouse/Agrp-IRES-Cre | Jackson Laboratories, Tong et al., 2008 (PMID:19160495) | CAT# 012899; RRID:IMSR_JAX:012899 |
| Mouse/Trh-I RES-Cre | Jackson Laboratories, Krashes et al., 2014 (PMID: 24487620) | Cat# 032468; RRID:IMSR_JAX:032468 |
| Mouse/Npy-hrGFP | Jackson Laboratories, van den Pol et al., 2009 (PMID:19357287) | Cat# 006417; RRID:IMSR_JAX:006417 |
| Mouse/Pomc-eGFP | Jackson Laboratories, Cowley et al., 2001 (PMID: 11373681) | Cat# 009593; RRID:IMSR_JAX:009593 |
| Oligonucleotides | ||
| Mm-Slc6a3-C3 | Advanced Cell Diagnostics | Cat#: 315441-C3 |
| Mm-Ghrh-C3 | Advanced Cell Diagnostics | Cat#: 470991-C3 |
| Mm-Trh | Advanced Cell Diagnostics | Cat#: 436811 |
| Mm-Pomc-C2 | Advanced Cell Diagnostics | Cat #: 314081-C2 |
| Recombinant DNA | ||
| Software and algorithms | ||
| Cell Ranger v5.0 | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger; RRID:SCR_017344 |
| bcl2fastq v2.20.0 | Illumina | https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html RRID: SCR_015058 |
| R | version 4.2.3 | https://www.r-proiect.org/ RRID:SCR_001905 |
| Seurat v4.3.0 | Stuart et al., 2019 | https://github.com/satiialab/seurat RRID:SCR_016341 |
| Seurat code used from cell clustering | Zenodo | |
| Illustrator | Adobe | https://www.adobe.com; RRID:SCR_010279 |
| Excel | Microsoft | https://www.microsoft.com/en-us/; RRID:SCR_016137 |
| ImageJ | version 2.9.0 | https://imagej.net/software/fiji/ RRID:SCR_003070 |
| Mouseland Cellpose | version 2.2.2 | https://github.com/MouseLand/cellpose |
| FED3 Software | version 1.16.3 | https://github.com/KravitzLabDevices/FED3_library |
| Other | ||
| FED3 devices | Matikainen-Ankney et al., 2021 | https://github.com/KravitzLabDevices/FED3 |