Key Resources Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
E. coli, OP50 strain	http://www.cgc.umn.edu	RRID: WB-STRAIN:OP50
Chemicals, Peptides, and Recombinant Proteins
Levamisole	http://www.acros.com	Cat # 187870100
Agarose	https://www.sigmaaldrich.com/	Cat # A9539
Experimental Models: Organisms/Strains
Strain name	Genotype	Reference
C. elegans Strain PT621: him-5(e1490) myIs4 [PKD-2::GFP+Punc-122::GFP]V	Bae et al (2006)40	RRID: WB-STRAIN: PT621
PT3716: unc-13(e51)I; him-5(e1490), myIs4V	this paper	RRID: WB-STRAIN: PT3716
PT3038: unc-31(e169)IV; him-5(e1490), myIs4V	This paper	RRID: WB-STRAIN: PT3038
PT443: myIs1[PKD2::GFP] pkd-2(sy606) IV; him-5(e1490)	Bae et al (200640)	RRID: WB-STRAIN: PT443
PT1195: klp-6(my8) III; him-5 (e1490), myIs4 V	Morsci and Barr (2011)41	RRID: WB-STRAIN: PT1195
PT835: pkd-2(sy606) myIs1 IV; nphp-4(tm925) him-5(e1490) V	Jauregui et al (2008)16	RRID: WB-STRAIN: PT835
PT3502: ttm-5(6585) I; him-5 (e1490), myIs4 V	This paper	RRID: WB-STRAIN: PT3502
PT3555: ttm-5(my92[TTM-5::GFP]) I; him-5(e1490)V	This paper	RRID: WB-STRAIN:PT3555
PT4108: ttm-5(my92[TTM-5::GFP]) I; lov-1(my78[lov-1::mSC])II; him-5(e1490)V	This paper	RRID: WB-STRAIN: PT4108
PT3629: lov-1(my78[lov-1::mSC])II; him-5(e1490)V	Walsh et al (2022)27	RRID: WB-STRAIN: PT3629
Software and Algorithms
Zen Blue 2.0	https://www.zeiss.com	N/A
Zen Black 2.0	https://www.zeiss.com	N/A
Imaris 9.5	https://imaris.oxinst.com	N/A
Prism 9	http://www.graphpad.com	N/A
Oligonucleotides
5′- GTTTGATCTCAACAATAACAGCAAGAAGCTCAAC GGAGGTGGCGGATCTGGAGGTGGAGGCTCTGGA GGAGGTGGATCTAGTAAAGGAGAAGAACTTTTC - 3′	This paper	TTM-5::GFP forward homology donor Primer
5′- gtttaaaagagaacatctgtatttaatccttcccaaaCTATTTG TATAGTTCATCCATG – 3′	This paper	TTM-5::GFP reverse homology donor Primer
5′ - AGCAAGAAGCUCAACUAGUU –3′	This paper	crRNA target sequence