KEY RESOURCES TABLE.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit anti-ARR3 | Millipore Sigma | Cat#AB15282; RRID:AB_1163387 |
| Goat anti Bhlhb5 | Santa Cruz | Cat#sc-6045; RRID:AB_2065343 |
| Mouse anti-CTBP2 | BD Biosciences | Cat#612044; RRID:AB_399431 |
| Rabbit anti-dsRed | Clontech | Cat#632496; RRID:AB_10013483 |
| Mouse anti FAT3 | 6,7 | N/A; RRID:AB_2904260 |
| Chicken anti GFP | Aves | Cat#GFP-1020; RRID:AB_10000240 |
| Mouse anti-GRIK1 (GluR5) | Santa Cruz Biotechnology | Cat#sc-393420; RRID:AB_2716684 |
| Sheep anti-GRM6 | 56 | N/A |
| Goat anti-PTPσ | R&D Systems | Cat#AF3430; RRID:AB_2175157 |
| Mouse anti-PTPσ | Medimabs | Cat# MM-0020-P |
| Rabbit anti VGAT | Synaptic systems | Cat#131002; RRID:AB_887871 |
| Mouse anti-VSX2 (Chx10) | Santa Cruz Biotechnology | Cat#sc-365519; RRID:AB_10842442 |
| Donkey anti chicken, Alexa Fluor® 488 | Jackson ImmunoResearch |
Cat#703-545-155; RRID:AB_2340375 |
| Donkey anti goat, Alexa Fluor® 568 | Thermo Fisher Scientific | Cat#A11057; RRID:AB_142581 |
| Donkey anti mouse, Alexa Fluor® 488 | Abcam | Cat#ab150105; RRID:AB_2732856 |
| Donkey anti mouse, Alexa Fluor® 568 | Thermo Fisher Scientific | Cat#A10037; RRID:AB_2534013 |
| Goat anti mouse, Alexa Fluor® 647 | Thermo Fisher Scientific | Cat#A-21235; RRID:AB_2535804 |
| Donkey anti rabbit, Alexa Fluor® 488 | Thermo Fisher Scientific | Cat#A21206; RRID:AB_2535792 |
| Donkey anti rabbit, Alexa Fluor® 568 | Thermo Fisher Scientific | Cat#A10042; RRID:AB_2534017 |
| Donkey anti rabbit, Alexa Fluor® 647 | Thermo Fisher Scientific | Cat#A31573; RRID:AB_2536183 |
| Donkey anti sheep, Alexa Fluor® 568 | Thermo Fisher Scientific | Cat#A-21099; RRID:AB_2535753 |
| Goat anti mouse - HRP | BioRad | Cat# 170-6516; RRID:AB_11125547 |
| Commercial assays | ||
| RNAscope® Multiplex | ACD | Cat#323100 |
| Fluorescent Reagent Kit v2 | ||
| HCR IHC Bundle | Molecular Instruments |
N/A |
| Experimental models: Organisms/Strains | ||
| Mouse: Ptf1aCRE | C. Wright, Vanderbilt U 19 | MGI:2387812 |
| Mouse: Isl1CRE | The Jackson Laboratory53 | Strain #: 024242 |
| Mouse: Bhlhe22CRE | M.E. Greenberg (Harvard Medical School)54 | N/A |
| Mouse: Fat3floxed | 7 | N/A |
| Mouse: Fat3ΔTM | 7 | N/A |
| Mouse: Fat3ΔICD-GFP | 6 | N/A |
| Mouse: Fezf2−/− | 49 | N/A |
| Mouse: Grikl−/− | Christophe Mulle (University of Bordeaux, France)50 | N/A |
| Mouse: Grm6−/− | The Jackson Laboratory51 | Strain #: 016883 |
| Mouse: Ptprs−/− | Michel Tremblay52 | MGI:2158757 |
| In situ hybridization Probes | ||
| RNAscope® Probe-Mm-Fat3-O1 | ACD | Cat# 509051 |
| RNAscope® Probe-Mm-Grik1-C3 | ACD | Cat# 438771-C3 |
| Recombinant DNA | ||
| AAV-Grik1-GFP plasmid | This paper | N/A |
| Viral vectors | ||
| AAV8-Grik1-GFP | This paper | N/A |