Figure 1.

Yeast isolates tested for amylolytic activity. PH-Gra1 was inoculated onto nutrient agar with 0.2% starch. Lugol solution was used to visualize the region of starch that had been hydrolyzed by amylolytic activity (“before” = prior to Lugol solution treatment; “after” = after Lugol solution treatment). Amylolytic activity was determined by the presence of a light purple region (indicated as white arrows) around the colony against a navy background. Commercial amylase was inoculated on the same medium as a positive control (+). Sterile H₂O and a non-amylase-producing strain were included as negative controls (−).