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[Preprint]. 2023 Oct 28:rs.3.rs-3127017. [Version 1] doi: 10.21203/rs.3.rs-3127017/v1

Figure 3. Anatomic protocol: Differentiation and Maturation (Ctrl2). A. Differentiation:

Figure 3

Phase contrast images display single cell seeding of iPSCs on DIV0 of differentiation and generation of immature neurons by DIV7. Scale bar DIV0–6 1000μm, DIV7 200μm. B. Maturation:Maturation of neurons DIV7–35. Differentiation resulted in a homogenous neuronal network during the maturation period. Scale Bar 200μm. C. Immunostaining of neurons for Peripherin and Tuj1 on DIV8, 14, 21, 28 and 35. Scale Bar DIV8 and 14 – 100μm, DIV21, 28, 35 – 200μm. DIV-Days in vitro. Peripherin-green, Tuj1-red and DAPI-blue fluorescence.