Figure 7. Anatomic iPSC-derived sensory neurons can be adapted for FLIPR^Penta high-throughput Ca²⁺ assays.

A. Ca²⁺ responses of sensory neurons at DIV15 plated with varying cell densities (1,000–10,000 cells/well) on 384-well plates. B. KCl-induced Ca²⁺ responses at DIV15, DIV21 and DIV35 C. sample trace from DIV21. D. Veratridine-induced Ca²⁺ responses at DIV15, 21 and 35 E. sample trace showing complete inhibition of responses by TTX (1 μM). F. Capsaicin-induced Ca²⁺ responses emerge at DIV35, G. capsaicin sample trace. H. 24 h treatment with IL6/soluble IL6-R does not affect the response properties of sensory neurons at DIV35. Data are presented as mean ±S.D. from n > 3 replicates.