Skip to main content
[Preprint]. 2023 Oct 28:rs.3.rs-3431502. [Version 1] doi: 10.21203/rs.3.rs-3431502/v1

Figure 4. Sex and subregional profiles of the Excitatory/lnhibitory (E/l) balance of the Insular Cortex.

Figure 4

(A-B) Schematic illustration of synaptic charge tranfered by each sEPSCs (A) and sIPSCs (B) calculated as the area inside each event as indicated by the arrows. (C) An analysis of the total charge of AMPA-sEPSCs measured over a 6-minute period across sexes revealed similar total charges in both male and female in both the alC and pIC. However, there was a higher total charge transfer observed in the alC compared with pIC, but this was only observed in males. (D) Similarly, the total charge of GABA-sIPSCs, when measured over a 6-minute period considering both area and sex, showed a comparable amount of charge transferred. This was analogous to the observations made in ‘C’ for AMPA-sEPSCs. (E-H)Cumulative frequency distribution of sEPSCs (E) and sIPSCs (I) total charge transfer obtain from each Insular cortex within male and female animals. Insets: dot plots and pie graph showing the proportion of E versus I extrapolated at P = 0.5 from the corresponding cumulative frequency. (C-D) Data are presented as box-and-whisker plots (minimum, maximum, median) and analyzed via two-way ANOVA followed by Šídák’s multiple comparison. P-values <0.05 are depicted in the graphs. (C)The sample sizes for alC male were 12/10 for pIC male 22/15, for alC female 13/10 and for pIC female 12/10. (D) The sample sizes for alC male were 19/11 for pIC male 13/9, for alC female 13/10 and for pIC female 16/10.