FIG. 2.

Physical-chemical parameters from aerobic ΔSterne-1(pPA102)CR4 fermentation. Symbols: open circles, DO₂; solid line, pH; open squares, agitation; solid squares, percent oxygen. The tryptone addition during the fermentation is indicated by the arrow above the percent oxygen data at 200 min. The temperature range was 36.7 to 37.4°C. Pressure was constant at 2.0 lb/in², and the sparge rate was 1 vol/vol/min. The sharp positive spike in the DO₂ values at 350 min was due to the addition of antifoam KFO673.