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SDS-PAGE analysis of PA purity after chromatographic purification steps. Lanes: 1, molecular weight standards; 2, PA after Macro-Prep 50 Q chromatography; 3, PA after HQ chromatography; 4, PA after QE chromatography; 5, PA after ether HIC chromatography. PA samples from each purification step were desalted, concentrated, and solubilized in SDS buffer. The total protein loaded in lanes 2 to 5 was 2 μg. Molecular weight standards were as in Fig. 4.
