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. 2023 Oct 30;19(10):e1011601. doi: 10.1371/journal.ppat.1011601

Fig 4. Molar mass and hydrodynamic radius of the CZA97.012 SOSIP.664 trimer measured by FFF-MALS.

Fig 4

A. 2G12-, PGT145-, or PGT151- and then SEC-purified trimer was separated by FFF, and the molar mass (g/mol) and hydrodynamic radius (Rh) measured by MALS. B. NS-EM was performed on CZA97.012 SOSIP.664 trimer purified with three different bNAbs. CZA97.012 SOSIP.664 trimer was purified by affinity chromatography with 2G12, PGT145, or PGT151 followed by SEC. The epitopes of the three bNAbs are shown in blue on the side and top views of the trimer. 2D-class averages are shown for each purification. The calculated proportions of native-like trimer are given in % below the NS-EM images.