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CZA97.012 SOSIP.664 trimer was purified on 2G12-, PGT145-, or PGT151-affinity columns and thereafter by SEC. Sensorgrams show binding to immobilized trimer (response units, RU) after background subtraction on the y-axis vs. time (s) on the x-axis. Note that the y axes have different scales in accordance with the maximum binding by each bNAb. Association was monitored for 300 s, dissociation for 600 s.
