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. 2023 Oct 30;19(10):e1011601. doi: 10.1371/journal.ppat.1011601

Fig 7. Site-specific glycosylation of differentially affinity-purified CZA97.012 SOSIP.664 trimer.

Fig 7

Trimer was purified by 2G12-, PGT145-, or PGT151-affinity chromatography followed by SEC. Differentially purified trimer was analyzed by a method based on PNGase F or Endo H digestion. The bar graphs represent the relative amounts of digested glycopeptides possessing the footprints for oligo-mannose- and hybrid-type glycans together (green), complex-type glycans (magenta), and unoccupied PNGS (gray). The results were obtained by LC-MS. The error bars represent s.e.m. of all peptides detected for each site; n.d. = not detectable (which does not suggest low occupancy).