Fig 9. Comparison of CZA97.012 SOSIP.664 trimer produced in different cell lines and differentially affinity-purified.

Trimer produced in either a stable CHO cell line or by transient transfection of HEK-293F cells was 2G12-, PGT145-, or PGT151-affinity- and then SEC-purified. Site-specific glycan content was determined by LC-MS on intact glycopeptides as in Fig 8B, but here the contents of oligomannose-type glycans were summed up, i.e., only Man₉GlcNAc₂, Man₈GlcNAc₂, Man₇GlcNAc₂, Man₆GlcNAc₂, and Man₅GlcNAc₂ together, and expressed as percentages of total glycan per site. The bar charts thus represent the relative accumulated oligomannose-type glycan content at each PNGS in CZA97.012 SOSIP.664. Light green represents CHO-derived and dark green HEK-293F-derived trimer (see legend). Sites for which glycopeptides of sufficient quality could not be obtained are left non-determined and are marked with asterisks.