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. 2023 Mar 6;41(11):1557–1566. doi: 10.1038/s41587-023-01685-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a) Representative distribution of reads per cell. (b) Correlation between reads and genes per cell. Spearman’s R and p values were calculated in R v4.1.0. (c,d) Comparison of UMIs/cell and genes/cell in current single-cell methods. Plots display a violin for a single representative sample for each platform. Transcripts per cell and genes per cell are separated by cell species (mouse (c) and human (d)), identified using the 85% species thresholding technique, as described in the methods. Box plots show the median, 25th and 75th percentiles. (e,f) Comparison of PIP-seq to 10X Genomics across a range of sequencing depths (0-80,000 reads/cell) (e) UMIs/cell and (f) genes/cell 80k cells down sampled from one biological replicate. Points represent the median with the lower and upper error bars corresponding to the 25th and 75th percentiles, respectively.