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. 2023 Nov 9;13:19456. doi: 10.1038/s41598-023-46859-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Many ANFs in the exposed ears contact the IHC but form no typical synaptic specializations. These 12 images span the entire region of IHC contact for one ANF from the 1-week survival case. Every 5th section is shown, as indicated by the numbers in the upper left: increasing numbers are closer to the top of the IHC. Dashed red circle indicates the possible prior location of the synaptic ribbon. Blue arrowheads in 31, 36 and 41 point to the prominent juxta-membranous cisterna present in the IHC. Contacts with two efferent terminals are visible, but only the regions indicated with red arrowheads from 6 to 16 were considered synaptic.