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. 2023 Sep 24;10(3):458–468. doi: 10.5455/javar.2023.j699

Table 2. Target genes, primer sequences, and amplicon sizes of different bacterial species.

Test target Target gene Sequences of primers (5’-3’) Amplified segment (bp) Reference
Salmonella spp. invA1 GTGAAATTATCGCCACGTTCGGGCAA 284 [25]
TCATCGCACCGTCAAAGGAACC
Clostridium spp. 16S rRNA 2 AAAGATGGCATCATCATTCAAC 360 [26]
TACCGTCATTATCTTCCCCAAA

invA1: invasion of the host epithelial cells (Conserved virulence gene), 16S rRNA2: Clostridium spp. Conserved gene.