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. 2023 Nov 9;14:7249. doi: 10.1038/s41467-023-43167-5

Fig. 5. Butyrate supplementation confers better preservation of cardiac function after myocardial injury.

Fig. 5

a Experimental design for the effects of butyrate on post-MI cardiac repair (upper panel) in SPF mice. Half of the mice were also treated with antibiotics (ABX) to deplete the gut microbiota. The relative bacterial load in feces on MI day 21 was determined with 16S rRNA qPCR (lower panel). b Level of fecal butyrate on MI day 21 using HPLC analysis. c Plasma level of β-hydroxybutyrate on MI day 21 using colorimetric assay. d–e Changes in left ventricular d EF and e FS in response to butyrate supplementation on MI day 21. Survival rate of SPF mice 21-days post-MI. f Representative images of cardiac infarct size labeled with picrosirus red and g quantification. h Catheterization analysis of post-MI cardiac function, including ESPVR, EDPVR, PRSW and dP/dt max (vs. EDV). The number of biologically independent mice are indicated in each chart. Data were analyzed with Kruskal–Wallis test followed by FDR correction. Data are represented as mean ± SEM.