Skip to main content
. 2023 Nov 9;14:7249. doi: 10.1038/s41467-023-43167-5

Fig. 7. Post-MI cardioprotection of butyrate-producers in hmgcs2-Δexon2 (HMGCS2-deficient/HMGCS2def) mice.

Fig. 7

a Schematic illustration of liver ketogenesis. b Schematic illustration of HMGCS2def mice using Cre-LoxP system (upper panel) and the level of HMGCS2 protein in liver (lower panel). c The plasma level of β-hydroxybutyrate in HMGCS2def mice before cardiac injury with colorimetric assay (Wt, n = 5; HMGCS2def, n = 8). d The schematic illustration of experimental design for butyrate-producing (Bp) bacteria transplantation in 16-week-old female HMGCS2def mice (upper panel) and the post-MI survival rate of HMGCS2def mice (lower panel). e Loading of Bifidobacterium adolescentis (B. adolescentis), Butyricimonas virosa (B. virosa) and Streptococcus parasanguinis (S. parasanguinis) in HMGCS2def stool. f The plasma level of β-hydroxybutyrate in HMGCS2def mice after MI determined with colorimetric assay. g–h Left ventricular ejection fraction (EF) (g) and fraction shortening (FS) (h) of HMGCS2def mice with/without butyrate-producer inoculation after MI. i Representative images of cardiac infarct size labeled with picrosirus red and statistics. The number of biologically independent mice are indicated in each chart. e–i Data were analyzed with the Kruskal–Wallis test followed by FDR correction. Data are represented as mean ± SEM.