TABLE 4.
Effect of various compounds on the oxidation of 2-nitropropane catalyzed by 2-nitropropane dioxygenase from N. crassa
| Additive | Concn (mM) | Relative activitya (%) |
|---|---|---|
| None | 100 | |
| Superoxide dismutase | 100 U | 0 |
| Cytochrome c | 0.04 | 34 |
| Nitroblue tetrazolium | 2.5 | 10 |
| EDTA | 1 | 95 |
| Iodoacetate | 1 | 0 |
| Dithiothreitol | 1 | 0 |
| 2-Mercaptoethanol | 1 | 2 |
| Glutathione (reduced form) | 5 | 28 |
a
The enzyme was assayed at 30°C for 5 min, as described in Materials and Methods, in a mixture containing the indicated concentration of each compound and 20 μmol of anionic 2-nitropropane in 1 ml of Britton-Robinson’s buffer (pH 6.5).