Figure 1.

Expression domains of Silc1 and Sox11

(A) Expression levels of Sox11 (blue) and Silc1 (gray) in sorted populations from different regions of the CNS. Data are from the Allen brain atlas. The bottom panel shows the different populations of cells in the hippocampus.

(B) Fluorescent in situ hybridization (FISH) assay using RNAscope for brain sections from a WT mouse. A negative control was performed in parallel as an indicator of background staining. Tissues were hybridized with Silc1 probe (red), counterstained with DAPI (blue), and imaged using a 100× oil immersion objective. Scale bar, 500 μm. Silc1 is expressed in the cortical area and in the hippocampus.

(C) Quantification of the indicated genes in RNA-seq data from the DG at the indicated time points following electroconvulsive stimulation using RNA-seq data from Su et al.²⁷ The p values were computed for comparison with the “0 h” time point using DeSeq2³⁷ and adjusted for multiple testing (n = 3).

See also Figure S1.