(A) Attachment and (B) entry. Vero cells were infected with CHIKV BRA/RJ/18 isolate at an MOI of 1.0 and compounds (100 μM) were added according to the superior scheme. Infected and untreated cells (VC) or infected cells treated with DMSO (0.2%, vehicle control) or chloroquine (CQ, 100 μM) were used as controls. At 24 hpi, virus titers were determined by plaque assays. Data are expressed as mean ± standard deviation (n = 3) and statistical analysis was carried out using ANOVA followed by the Tukey test. Statistical significance was assessed relative to the VC group (****p < 0.0001).
CHIKV: Chikungunya virus; COMPD: Compound; CQ: Chloroquine; DMSO: Dimethyl sulfoxide; VC: Virus control.