| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-GFP | Abcam | Cat#ab13970; RRID: AB_300798 |
| Guinea pig anti-(Arg8)-vasopressin | Peninsula Laboratories | Cat#T5048; RRID: AB_518680 |
| Mouse anti-OT-neurophysin | Dr. Harold Gainer | PS38 |
| Rabbit anti-C-CASP3 (Asp175) | Cell Signaling | Cat#9661; RRID: AB_2341188 |
| Rabbit anti-c-fos | Synaptic Systems | Cat#226003; RRID: AB_2891278 |
| Rabbit anti-dsRed (Living Colors®) | Takara Bio | Cat#632496; RRID: AB_10013483 |
| Alexa Fluor 405 goat anti-mouse IgG | Invitrogen | Cat#A31553 |
| Alexa Fluor 488 goat anti-chicken IgG | Invitrogen | Cat#A11039 |
| Alexa Fluor 568 donkey anti-rabbit IgG | Invitrogen | Cat#A10042 |
| Alexa Fluor 647 donkey anti-goat IgG | Invitrogen | Cat#A21447 |
| Alexa Fluor 647 donkey anti-rabbit IgG | Invitrogen | Cat#A31573 |
| Alexa Fluor 647 donkey anti-sheep IgG | Invitrogen | Cat#A21448 |
| Chemicals, peptides, and recombinant proteins | ||
| 4′, 6-Diamidino-2-phenylindol (DAPI) | Thermo Fisher | Cat#1306; CAS No. 28718-90-3 |
| 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) | Sigma-Aldrich | Cat#C239; CAS No. 115066-14-3 |
| A-71623 (CCKAR agonist) | Tocris | Cat#2411/1; Cas No. 130408-77-4 |
| Bovine serum albumin | Sigma-Aldrich | Cat#A8806; CAS No. 9048-46-8 |
| Calbiochem® Phosphatase and Protease Inhibitors | Merck Millipore | Cat#134544 |
| Cholecystokinin octapeptide, sulfated | Tocris | Cat#1166; CAS No. 25126-32-3 |
| Clozapine-N-oxide | Enzo | Cat#BML-NS105-0025; CAS No. 34233-69-7 |
| D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5) | Tocris | Cat#0106; CAS No. 79055-68-8 |
| Deoxycholate | Thermo Fisher | Cat#89005; CAS No. 302-95-4 |
| Dibenzyl ether (DBE) | Sigma-Aldrich | Cat#33630; CAS No. 103-50-4 |
| Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | Cat#276855; CAS No. 67-68-5 |
| DL-Dithiothreitol (DTT) | Sigma-Aldrich | Cat#D8255; CAS No. 578517 |
| Fluorogold | Santa Cruz | Cat#sc-358883; CAS No. 223769-64-0 |
| Gelatin from porcine skin (SUMI) | VWR International | Cat#SAFSG1890; CAS No. 9000-70-8 |
| Glycine | Sigma-Aldrich | Cat#G7126; CAS No. 56-40-6 |
| Heparin sodium | Sigma-Aldrich | Cat#H0200000; CAS No. 904108-1 |
| IPEGAL CR-630 | Sigma-Aldrich | Cat#18896; CAS No. 9002-93-1 |
| Methanol | Sigma-Aldrich | Cat#322415; CAS No. 67-56-1 |
| Mowiol® 4-88 | Merck Milllipore | Cat#81381-250G; CAS No. 81381 |
| NeuroTrace™ 435/455 | Thermo Fisher | Cat#N21479 |
| Nonidet™ P40 substitute | Sigma-Aldrich | Cat#74385; CAS No. 9016-45-9 |
| Paraformaldehyde | Carl Roth | Cat#0335.2; CAS No. 30525-89-4 |
| Picrotoxin | Sigma-Aldrich | Cat#P1675; CAS No. 124-87-8 |
| Pierce™ Horsereadish peroxidase | Thermo Fisher | Cat#31490; CAS No. 9003-99-3 |
| Potassium chloride (KCl) | Sigma-Aldrich | Cat#P3911-25G; CAS No. 7447-40-7 |
| Primers | Thermo Fisher | N/A |
| RNasin Plus RNase inhibitor | Takara Clontech | Cat#2313A |
| Spermidine | Sigma-Aldrich | Cat#85553; CAS No. 124-20-9 |
| Spermine | Sigma-Aldrich | Cat#S3256; CAS No. 71-44-3 |
| SuperAsein RNase Inhibitor | Thermo Fisher | Cat#AM2696 |
| Syntocinon (Oxytocin) | Selleckchem | Cat#P1902; CAS No. 50-56-6 |
| Tamoxifen | Sigma-Aldrich | Cat#T5648; CAS No. 10540-29-1 |
| TaqMan Gene Expression Assay | Thermo Fisher | N/A |
| TaqMan Universal Master Mix II, no UNG | Thermo Fisher | Cat#4440040 |
| Triton X-100 | Roche Diagnostics | Cat#11858620; CAS No.9036-19-5 |
| Tween 20 | Sigma-Aldrich | Cat#P1379; CAS No. 9005-64-5 |
| Critical commercial assays | ||
| Agilent RNA 6000 Pico Kit | Agilent Technologies | Cat#5067-1513 |
| Quant-IT RiboGreen Kit | Thermo Fisher | Cat#Q33210 |
| RNeasy Micro Kit | QUIAGEN | Cat#74004 |
| RNeasy Mini Kit | QUIAGEN | Cat#74104 |
| SMART-Seq® v4 Ultra® Low Input RNA Kit | TaKaRa | Cat#6344893 |
| SMARTer® PCR cDNA synthesis kit | TaKaRa | Cat#634455 |
| Tetramethyl benzidine substrate (TMB) | BioLegend | Cat#421101 |
| Deposited data | ||
| snRNA-seq2 (raw data; GEO - NCBI) | This paper | GSE243448 |
| RiboTag-mRNA-seq (raw data; GEO – NCBI) | This paper | GSE242245 |
| RiboTag-mRNA-seq and snRNA-seq2 - code (Zenodo) | Zenodo | https://doi.org/10.5281/zenodo.7673205; https://zenodo.org/record/7673205#.Y_h_bHbMI2x |
| RiboTag-mRNA-seq and snRNA-seq2 - code (Github) | Github | https://github.com/viktormiok/snRNAseq_RiboTagRNAseq-oxytocin |
| Experimental models: Organisms/strains | ||
| Ai14 (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) | Jackson Laboratory | RRID: IMSR_JAX:007914 |
| CAG-Sun1sfGFP (B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J) | Jackson Laboratory | RRID: IMSR_JAX:021039 |
| Oxtrflox(B6.129(SJL)-Oxtrtm1.1Wsy/J) | Jackson Laboratory | RRID: IMSR_JAX:008471 |
| Oxytocin-ires-Cre (B6;129S-Oxttm1.1(cre)Dolsn/J) | Jackson Laboratory | RRID: IMSR_JAX:024234 |
| RiboTag (B6N.129-Rpl22tm1.1Psam/J) | Jackson Laboratory | RRID: IMSR_JAX:011029 |
| ROSA-DTA (B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J) | Jackson Laboratory | RRID: IMSR_JAX:009669 |
| ROSA.CreERT2(B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J) | Jackson Laboratory | RRID: IMSR_JAX:008463 |
| Oligonucleotides | ||
| Cckar (TaqMan probe) | Thermo Fisher | Mm00438060 |
| Cckbr (TaqMan probe) | Thermo Fisher | Mm00432329 |
| Hprt (TaqMan probe) | Thermo Fisher | Mm01545399 |
| Mc4R (TaqMan probe) | Thermo Fisher | Mm00457486 |
| Ot (TaqMan probe) | Thermo Fisher | Mm01329577 |
| Otr (TaqMan probe) | Thermo Fisher | Mm01182684 |
| Sim1 (TaqMan probe) | Thermo Fisher | Mm00441390 |
| Software and algorithms | ||
| FIJI | NIH | https://ImageJ.net/Fiji/Downloads |
| FlowJo | BD | N/A |
| GraphPad Prism 9.4.1 | GraphPad Software | N/A |
| IMARIS ×64 9.1.2 and ×64 9.2 | Bitplane, Oxford Instruments | N/A |
| MacLab Chart Pro 7.0 | GE Healthcare | N/A |
| RStudio 4.21 | Posit, RStudio PBC | https://www.rstudio.com/products/rstudio/download/ |
| Other | ||
| AAV2/1-DIO-CAG-GCaMP6f | (Chen et al.)98 | Addgene viral prep #100839 |
| AAV2/1-DIO-hSYN1-hM3Dq-mCherry | (Krashes et al.)99 | Addgene viral prep #44361 |
| AAV2/1-DIO-hSYN1-mCherry | Bryan Roth lab (unpublished) | Addgene viral prep #50459 |
| AAV2/1-OTp-iCre | Custom made23 | N/A |
| AAV2/1-OTp-Venus | Custom made23 | N/A |
| Ketamine (Ketabel) | HFW/Bremer Pharma GmBH | CAS No. 6740-88-1 |
| Meloxicam | WDT/Pharma-Partner-Vetriebs GmBH | CAS No. 71125-38-7 |
| Metamizol | WDT/Pharma-Partner-Vetriebs GmBH | CAS No. 68-89-3 |
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