Figure 4.

FBP1 S170 phosphorylation inhibits tumor growth and is negatively associated with poor prognosis in NSCLC patients. A. A549 cells (4 × 10⁶) with reconstituted expression of different FBP1 mutants were subcutaneously injected into the flank region of athymic nude mice (n=6 per group). The resulting tumors were resected 28 days after injection. A representative image of tumor sizes is shown. B. The tumors in the mice were weighed. The data represent the mean ± SD of 6 mice. ****P < 0.0001. C. IHC analyses of the tumor tissues were performed. Representative images are shown. D. Representative IHC staining of OGT and FBP1 pS170 in NSCLC and adjacent nontumor tissues is shown. The expression levels of OGT and FBP1 pS170 in NSCLC and adjacent nontumor tissues were quantified and compared between NSCLC and adjacent nontumor tissues. ****P < 0.0001. E. Representative IHC staining of OGT and FBP1 pS170 in NSCLC tissues is shown. The correlations between the expression levels of OGT and FBP1 pS170 were analyzed. F. Analysis of the expression levels of OGT and FBP1 pS170 with the prognosis of NSCLC patients was performed.