Figure 2.

Effect of SDF1 on HUVEC migration in vitro. Migrated HUVEC were stained with Harris Hematoxylin in transwell membranes and quantified after 8 h of stimulation with SDF1. (A) Representative pictures that show the migrated cells after being cultured in SFM (negative control), a stimulation medium with free human SDF1 at 20 ng/mL (in SFM), a stimulation medium with human SDF1 released from CH at 20 ng/mL (CH-SDF1), and a CoM (complete medium, positive control). (B) Fold increase in the number of migrated HUVEC in the different experimental groups compared to the nonstimulated cells (SFM = serum-free medium). Three independent experiments were performed in duplicate. Data are represented as mean ± SEM. Statistical significance: *P < 0.05, **P < 0.01, ****P < 0.0001.