Figure 4.

MELK is crucial for regulating E2F8 expression in prostate cancer cells. (A) Pearson correlation analysis showed coexpression correlation between MELK and E2F8 in TCGA-PRAD and cBioPortal transcriptomic data. (B) MELK expression levels in patients with prostate cancer (GSE3325) were represented in the box plots. ***P<0.005, n.s., not significant. (C) Each cell was transfected with 50 nM siMELK-1 or siMELK-2 for 48 h prior to western blot analysis. (D) Each cell was transfected with 50 nM siMELK-1 for 9 days (once every 3 days), after which colony formation assessed. The data were expressed as the mean ± SEM (n=3). **P<0.01; ***P<0.005. TCGA-PRAD, The Cancer Genome Atlas-Prostate Adenocarcinoma; r, Pearson correlation coefficient; si, short interfering.