Extended Data Fig. 4 |. WGP-induced training modulates T cells and BM progenitors.

a, PBS (n = 9) or WGP-trained (n = 10) mice were challenged with 0.4 × 10⁶ LLC cells intravenously. Mice were enthunized at day 16 for T cell phenotype analysis. Percentage of FoxP3⁺ Tregs, TNF expressing CD4⁺ and CD8⁺ T cells in the lungs from PBS vs WGP-trained mice. Data are representative of two independent experiments pooled together. b, Mice were trained with or without WGP (n = 5) for 7 days and then challenged with 0.4 × 10⁶ EL4 lymphoma cells i.v. followed by euthanizing at day 16 post tumor cell injection. Representative liver micrographs, summarized number of liver nodules, and liver weights of PBS and WGP-trained EL4 tumor cell-bearing mice are shown. c, CCR2 expression on lung IMs and intracellular TNF production in CCR2⁺ IMs from mice reconstituted with BM cells from WGP-trained or PBS control mice (n = 5). Representative dot plots and summarized data from one of two independent experiments are shown. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001. P values were derived from unpaired two-tailed student’s t-test for a, b and c.