Extended Data Fig. 5 |. Macrophages are the effector cells that control metastasis.

a, Depletion efficiency of lung IM and spleenic macrophages after Clodronate liposome (Clodrosome) injection. b, Summarized data for percentage of LLC-GFP cells in WGP-trained and untrained WT and CCR2 KO mice. WT and CCR2 KO mice were trained with PBS or WGP for a week followed by challenge with i.v. GFP-LLC cells (n = 5). Mice were then euthanized to analyze the tumor burden in the lungs 2 weeks later by flow cytometry. c, Schema for in vivo Ly6G depletion, WGP training, and tumor challenge protocol (n = 5, 5, 4, 5). d, Depletion efficiency of lung Ly6G⁺ neutrophils after weekly injections with Ly6G depletion antibody or isotype antibody by flow cytometry. e, Summarized data for percentage of LLC-GFP cells in neutrophil depleted or isotype antibody treated WGP trained and untrained mice. f, Depletion efficiency of CD4 and CD8 T cells in the lungs after CD4 and CD8 depletion antibody or isotype antibody injection. g, Representative dot plots and summarized data for intracellular TNF expression on lung IM after ex vivo LPS re-stimulation of PBS versus WGP-trained NSG mice (n = 4). h, Representative dot plots and summarized data for tumor burden in the lungs of WGP trained and untrained NSG mice. NSG mice trained with PBS (n = 7) or WGP (n = 9) were challenged with GFP-LLC cells i.v. and the lungs were harvested 48 h later to analyze the tumor burden in the lungs by flow cytometry. Data are representative of one of two independent experiments and presented as mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001. P values were derived from two way ANOVA with Tukey’s multiple comparison test for b, one way ANOVA with Tukey’s multiple comparison test for e, unpaired two-tailed student’s t-test for g and h.