Figure 3.

pHP1α phase separation slows the dynamics of the H3 and H4 tails. (a and b) Comparison of 1D ¹H–¹³C INEPT MAS NMR experiments of samples containing mononucleosomes prepared with (a) ¹⁵N,¹³C-labeled H3 or (b) ¹⁵N,¹³C-labeled H4 in the presence or absence of pHP1α. (c and d) 2D ¹H–¹³C INEPT MAS NMR experiments of the same samples. (e and f) Analysis of the peak intensity ratios of the 2D INEPT experiments in the presence (I_bound) and absence (I_free) of pHP1α. I_bound was corrected for the contribution of natural abundance pHP1α as described in the Experimental Section and Figure S10.