Skip to main content
. 2023 Jun 30;115(11):1392–1403. doi: 10.1093/jnci/djad126

Figure 1.

Figure 1.

Programmed death ligand 1 (PD-L1) cancer cell-intrinsic phenotypes. A) Immunoblot confirming PD-L1 overexpression. B) PD-L1 overexpression and recombinant programmed cell death protein 1 (rPD-1) increase invasion. C) PD-L1 overexpression decreases time to scratch-wound closure, exacerbated by rPD-1. (Wound confluence 16 hours postscratch: CUHN013 empty = 60.95%; empty rPD-1 = 83.85%; PD-L1 overexpression = 85.59%; PD-L1 overexpression rPD-1 = 94.09%; CUHN036 empty = 68.74%; empty rPD-1 = 81.36%; PD-L1 overexpression = 87.51%; PD-L1 overexpression rPD-1 = 97.08%.) D) PD-L1 overrexpression increases clonogenicity. E) Immunoblot confirming short hairpin RNA knockdown of PD-L1 (shPD-L1). F) shPD-L1 decreases invasion, and rPD-1 is not sufficient to rescue the decrease. G) shPD-L1 slows scratch-wound closure and is not rescued by rPD-1 (wound confluence 16 hours postscratch: CUHN013 shC = 80.46%; shC rPD-1 = 91.87%; sh1 = 57.89%; sh1 rPD-1 = 55.76%; sh2 = 64.34%; sh2 rPD-1 = 51.19%; CUHN036 shC = 99.65%; shC rPD-1 = 100%; sh1 = 87.89%; sh1 rPD-1 = 93.12%; sh2 = 87.88%; sh2 rPD-1 = 88.75%). H) shPD-L1 decreases clonogenicity. rPD-1 has no significant effect on clonogenic growth. PD-L1 overexpression invasion and clonogenic measurements are normalized to empty control, shPD-L1 measurements are normalized to shCTRL. *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .0001. Error bars represent standard deviation. E = empty; FC = fold change; OE = PD-L1 overexpression; P = parental; shC = short hairpin RNA non-targeting control; sh1 = short hairpin RNA targeting PD-L1 #1; sh2 = short hairpin RNA targeting PD-L1 #2; rPD-1 = recombinant PD-1.