Skip to main content
. 2023 Jun 30;115(11):1392–1403. doi: 10.1093/jnci/djad126

Figure 5.

Figure 5.

Recombinant programmed cell death protein 1 (rPD-1) in vivo. A) rPD-1 is stable in vivo for 2 weeks as measured by enzyme-linked immunosorbent assay of NOD-scid-gamma (NSG) blood (n = 3). B-C) rPD-1 increased immunohistochemistry expression of programmed death ligand 1 (PD-L1), interleukin enhancer binding factor 2 (ILF2), interleukin enhancer binding factor 3 (ILF3), and phospho–signal transducer and activator of transcription 3 (STAT3) but did not alter expression of total STAT3. D) Proximity ligation assay confirms in vivo interaction between PD-L1 and rPD-1, ILF2, ILF3, and STAT3 in CUHN013 in vivo. *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .0001. Error bars represent standard deviation. Scale bar = 10μm (left); magnified PLA (right) is 4x original size. p-STAT = phospho-STAT3.