TABLE 1.
Bacterial strains and plasmids
| E. coli strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| B | Wild type | ATCC 11303b |
| ER2275 | trp31 his1 tonA2 rpsL104 supE44 xyl-7 mtl-2 metB1 e14+ Δ(lac)U169 endA1 recA1 R(zgb-210::Tn10) Tets Δ(mcr-hsd-mrr)114::IS10/F′ proAB lacI ZΔM15zz::min-Tn10 (Kmr) | NEBc |
| MC1060 | F− Δ(lacI-lacY)74 galE15 galK16 relA1 rpsL150 spoT1 hsdR2 λ− | CGSC 6648d |
| Plasmids | ||
| pUC19 | 2.6 kb; Ampr | 31 |
| pSOS95 | 7.0 kb; Ampr Emradc ctfA ctfB | 24a |
| pTECO11 | 7.4 kb; Amprthl | 20 |
| ppACT | 4.8 kb; Ampradc ctfA ctfB | This study |
| pACT | 6.2 kb; Amprthl adc ctfA ctfB | This study |
a
Abbreviations: adc, AADC gene; ctfA and ctfB, genes for the acetoacetyl-CoA:acetate/butyrate:CoAT subunits; recA, homologous recombination abolished; lacZ, β-galactosidase gene; mcrBC, methylcytosine-specific restriction system; thl, thiolase gene; Ampr, ampicillin resistant.
b
From American Type Culture Collection, Rockville, Md.
c
NEB, New England BioLabs, Beverly, Mass.
d
From the E. coli Genetic Stock Center.