Figure 4.

LEAWBIH promoted cell proliferation, migration, and invasion in an m⁶A dependent manner. (A and B) LEAWBIH expression in HuH-7 (A) and SK-HEP-1 (B) cells with stable overexpression of wild-type or m⁶A-modified 2095 and 2679 sites mutated LEAWBIH was detected by qPCR. (C and D) Cell viability of HuH-7 (C) and SK-HEP-1 (D) cells with overexpression of wild-type or mutated LEAWBIH was measured by Glo cell viability assay. (E) Cell proliferation of HuH-7 and SK-HEP-1 cells with overexpression of wild-type or mutated LEAWBIH was measured by EdU incorporation assay. Scale bars, 200 µm. (F) Cell migration of HuH-7 and SK-HEP-1 cells with overexpression of wild-type or mutated LEAWBIH was measured by transwell migration assay. Scale bars, 100 µm. (G) Cell invasion of HuH-7 and SK-HEP-1 cells with overexpression of wild-type or mutated LEAWBIH was measured by transwell invasion assay. Scale bars, 100 µm. Results are presented as mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001, ns, not significant, by one-way ANOVA followed by Dunnett’s multiple comparisons test.