. 2023 Oct 26;14:1280289. doi: 10.3389/fphar.2023.1280289

TABLE 1.

Estimated LD₂₀, LD₅₀, and LD₈₀ values (±95% CL) for xylazine, fentanyl, and fentanyl/xylazine combinations in male and female mice.

	Xylazine alone	Xylazine +56 fentanyl	Fentanyl alone	Fentanyl +100 xylazine
Males and females
LD₂₀	139.7 (122.9–158.8)	16.5 (12.0–22.9) ^a	99.3 (81.5–120.9)	0.26 (0.15–0.44) ^a
LD₅₀	157.2 (138.2–178.7)	32.0 (23.2–44.1) ^a	131.3 (107.8–159.9)	1.27 (0.74–2.16) ^a
LD₈₀	176.9 (155.5–201.1)	61.8 (44.7–85.2) ^a	173.5 (142.5–211.3)	6.20 (3.64–10.56) ^a
Males only
LD₂₀	137.9 (120.6–157.7)	8.0 (5.1–12.5) ^a	96.4 (79.9–116.2)	0.12 (0.06–0.25) ^a
LD₅₀	156.1 (136.5–178.5)	21.8 (13.9–34.3) ^a	122.4 (101.5–147.5)	1.09 (0.55–2.18) ^a
LD₈₀	176.7 (154.5–202.1)	59.7 (38.0–93.7) ^a	155.4 (128.9–187.4)	9.60 (4.80–19.19) ^a
Females only
LD₂₀	137.9 (120.6–157.7)	32.9 (26.2–41.6) ^a ^, ^b	97.4 (78.4–121.1)	0.30 (0.19–0.63) ^a
LD₅₀	156.1 (136.5–178.5)	51.6 (40.9–65.0) ^a ^, ^b	138.1 (111.1–171.7)	1.43 (0.93–2.20) ^a
LD₈₀	176.7 (154.5–202.1)	80.6 (64.0–101.6) ^a	195.8 (157.6–243.4)	6.87 (4.46–10.58) ^a

Note: All values expressed in mg/kg.

^{^a}

Indicate significant difference from drug alone.

^{^b}

Indicate significant difference from males.