TABLE 3.

Reported kinetic values for degradation of TCE by methanotrophs known to be expressing either sMMO or pMMO

Straina	Enzyme	Copper concn (μM)	Vmax (nmol/min/mg of protein)b	Ks (μM)b	Vmax/Ks (liters/min/mg of protein)	Reference
BB5.1	pMMO	28	0.10–0.15c	8–10c	1.2 × 10−5–1.5 × 10−5c	35
BB5.1	pMMO	3	NMd	NM	NM	35
OB3b	pMMO	20	2.5–4.1	7.9–36	6.9 × 10−5–5.2 × 10−4	This study
OB3b	pMMO	2.5	NM	NM	NM	This study
OB3b	pMMO	1.0	NM	NM	NM	6
OB3b	pMMO	4.8	<0.20e	NDf	ND	26
OB3b	pMMO	2.5g	0.296	ND	ND	10
A45	pMMO	2.5g	0.233	ND	ND	10
MN	pMMO	2.5g	0.677	ND	ND	10
OBBP	pMMO	2.5g	0.202	ND	ND	10
MC	pMMO	2.5g	0.58	ND	ND	10
OB3b	sMMO	0h	54–300e	ND	ND	26
OB3b	sMMO	0h	456	138	3.3 × 10−3	6
OB3b	sMMO	0h	995 (160)	126 (8)	7.9 × 10−3	19
OB3b	sMMO	0h	580 (200)e	145 (61)	4 × 10−3	27
68-1	sMMO	0h	2,235 (260)	225 (13)	9.9 × 10−3	19

^aA45, Methylobacter marinus A45; BB5.1, Methylobacter sp. strain BB5.1; MN, Methylomonas strain MN; OBBP, Methylocystis parvus OBBP; MC, Methylococcus capsulatus Bath; OB3b, Methylosinus trichosporium OB3b; 68-1, Methylomonas methanica 68-1. 

^bNumbers in parentheses indicate the standard deviation of the reported value. 

^cIt should be noted that the units reported in reference 35 are incorrect but are shown here in the corrected form (20). 

^dNM, no measurable TCE degradation. 

^eConverted from reported units of nanomoles per minute per milligram of cells, assuming cell dry weight is 50% protein. 

^fND, not determined. 

^gCells grown with 2.5 μM copper, but TCE degradation assays performed with 20 μM copper in the reaction mixture. 

^hNo copper added to growth medium.