FIG. 3.

Growth (A₆₀₀) and relative POI area (obtained from HPLC chromatograms) as a function of time in a PP358 batch culture grown without copper. Symbols: ⧫, culture A₆₀₀; ○, relative POI area. (Inset) Relative POI area (▴) as a function of the first moment of bacterial growth (area under bacterial growth curve). The relative POI area is the area of the SE-HPLC peak at a 25-ml elution volume divided by a representative peak area derived from the PP358 cultures (A₆₀₀ = 1.06; 5 μM Cu). The line is a manual fit to the data. The experiment was repeated, and the same qualitative results were obtained. Abs, absorbance.