Table 2.
Primer sequences used for quantitative real-time PCR.
| Genesb | GenBank | Primer sequencesa, 5′-3′1 | Size, bp |
|---|---|---|---|
| β-actin | NM_205518.1 | F:ATTGTCCACCGCAAATGCTTC R:AAATAAAGCCATGCCAATCTCGTC | 113 |
| NRF2 | XM_015289381.2 | F:CCCGCACCATGGAGATCGAG | 180 |
| R:GGAGCTGCTCTTGTCTTTCCT | |||
| HO-1 | NM_205344.1 | F:GTCGTTGGCAAGAAGCATCC | 106 |
| R: GGGCCTTTTGGGCGATTTTC | |||
| SOD1 | NM_205064.2 | F:CGCAGGTGCTCACTTCAATCC R:CAGTCACATTGCCGAGGTCAC | 89 |
| SOD2 | NM_204211.2 | F:GCTGTATCAGTTGGTGTTCAAGGA R:GCAATGGAATGAGACCTGTTGTTC | 130 |
| CAT | NM_001031215.2 | F:GGAGGTAGAACAGATGGCGTATG R:CGATGTCTATGCGTGTCAGGAT | 114 |
| GPX1 | NM_001277853.3 | F:CGGCTTCAAACCCAACTTCAC R:CTCTCTCAGGAAGGCGAACAG | 85 |
| SIRT1 | XM_046920057.1 | F:GATCAGCAAAAGGCTGGATGGT | 143 |
| R:ACGAGCCGCTTTCGCTACTAC | |||
| CPT-1 | NM_001012898.1 | F:ACAGCGAATGAAAGCAGGGT | 93 |
| R:GCCATGGCTAAGGTTTTCGT | |||
| PPARα | NM_001001464.1 | F:AGTAAGCTCTCAGAAACTTTGTTG | 161 |
| R:AGGTTGAAACAGAAGCCGC | |||
| APOA1 | XM_046932309.1 | F:CGCATTCGGGATATGGTGGA | 102 |
| R:GTCAAGCTGTTTGCCCACAG | |||
| SREBP-1 | AY029224 | F: GCAGAAGAGCAAGTCCCTCAA | 104 |
| R: TCGGCATCTCCATCACCTC |
a
F, forward primer; R, reverse primer.
b
SIRT1, sirtuin1; NRF2, nuclear factor erythroid 2-related factor 2; HO-1, heme-oxygenase 1; SOD1, superoxide dismutase 1; SOD2, superoxide dismutase 2; CAT, catalase; GPX1, glutathione peroxidase 1; CPT-1, carnitine palmitoyl-transferase 1; PPARα, peroxisome proliferator-activated receptor α; APOA1, apolipoprotein A1; SREBP-1, sterol regulatory element-binding protein-1.