Table 1. Primer and shRNA sequences used in the present study.

Primer/shRNA name	Primer sequence	Enzyme
Primers for qRT-PCR
Human:
BKβ1 sense	5’-CTTCTCCGCACCTCGGGGGA-3’
BKβ1 antisense	5’-CGGTCAGCAGGAAGGTGGGC-3’
Rat:
BKβ1 sense	5′-ACCCATGCCTTTGGGTCAAT-3′
BKβ1 antisense	5′-ATAGAGGCGCTGGTACACAA-3′
GAPDH sense	5′- GCGAAAGCATTTGCCAAGAA-3′
GAPDH antisense	5′-GGCATCGTTTATGGTCGGAAC-3′
shRNA sequences
Human:
shBKβ1 sense	5’-ccggCCACCTGATTGAGACCAACATCTCG AGATGTTGGTCTCAATCAGGTGGTTTTTg-3’
shBKβ1 antisense	5’-aattcAAAAACCACCTGATTGAGACCAACAT CTCGAGATGTTGGTCTCAATCAGGTGG-3’
Rat:
shBKβ1 sense	5’-ccggGGACAACTACCAGACAGCCTTCTCG AGAAGGCTGTCTGGTAGTTGTCCTTTTTg-3’
shBKβ1 antisense	5’-aattcAAAAAGGACAACTACCAGACAGCCTT CTCGAGAAGGCTGTCTGGTAGTTGTCC-3’
Primers for KCNMB1 promoter construct
(-1,783/+98)KCNMB1 sense	5’-TATAGGTACCatccttggaaaattgctctt-3’	KpnI
(-647/+98)KCNMB1 sense	5’-TATAGGTACCcttcagctcagttggtcc -3’	KpnI
(-296/+98)KCNMB1 sense	5’-TATAGGTACCtttctggcatctttgccag-3’	KpnI
Antisense	5’-ATATAAGCTTtctcccatgtgccgtcctcc-3’	HindIII
Primers for KCNMB1 promoter site-directed mutagenesis
Binding site 1 mutation sense	5’-TGAGAGTGctgtctttcaattTGAGTGGC-3’
Binding site 1 mutation antisense	5’-GCCACTCAaattgaaagacagCACTCTCA-3’
Binding site 2 mutation sense	5’-TTGCTCAGttctgtgacaagtGTAAGTGC-3’
Binding site 2 mutation antisense	5’-GCACTTACacttgtcacagaaCTGAGCAA-3’
Primers used for ChIP in the KCNMB1 promoter
Distant region sense	5’- ATTCAGAGTATCACGAGCAA-3’
Distant region antisense	5’-CCACTCAGAAGCAGGAAA-3’
Binding site 1 sense	5’-GAAAGGGGTGGAGAAGGGC-3’
Binding site 1 antisense	5’-CGAGCTCACCACCTTCCTC-3’
Binding site 2 sense	5’-GAGGTGCCATAACTTGCT-3’
Binding site 2 antisense	5’-CCCTGGCATATCCACGCT-3’

Text in bold indicates the core sequence. ChIP, chromatin immunoprecipitation; sh/shRNA, short hairpin RNA