Figure 2.

Immune cell frequencies in the trans men cohort were unaffected by testosterone injections

(A) Automated leukocyte counts in the hemogram of longitudinal trans men cohort samples. Total counts per nL remained stable during the study period (R² = 0.58, p = 0.95).

(B) t-distributed stochastic neighbor embedding (t-SNE) plot of 106 immune cell population frequencies for all five time points in the trans men cohort (n = 10), as identified by flow cytometry. Samples from each donor form a separate cluster, showing stability between the different time points. Each symbol color represents an individual donor, and different shades indicate the different time points.

(C) Frequencies of pDCs, depicted from the identical dataset used in (B), did not change significantly over time (p = 0.15). Statistical significance in (A), (C) was as assessed by extra sum-of-squares F test comparing a linear regression model with a shared slope and individual intercepts to a respective model with a hypothetical slope of 0. n = 10.