REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TotalSeq™-C0251 anti-human Hashtag 1 Antibody	Biolegend	Cat#: 394661 / RRID:AB_2801031
TotalSeq™-C0252 anti-human Hashtag 2 Antibody	Biolegend	Cat#: 394663 / RRID:AB_2801032
TotalSeq™-C0253 anti-human Hashtag 3 Antibody	Biolegend	Cat#: 394665 / RRID:AB_2801033
TotalSeq™-C0254 anti-human Hashtag 4 Antibody	Biolegend	Cat#: 394667 / RRID:AB_2801034
BDCA2 (CD303) / PE-Cy7 / 201A	Biolegend	Cat#: 354214 / RRID: AB_2563141
CCR2 (CD192) / BV421 / K036C2	Biolegend	Cat#: 357210 / RRID:AB_2563463
CCR4 (CD194) / BV605 / L291H4	Biolegend	Cat#: 359418 / RRID:AB_2562483
CCR5 (CD195) / BV421 / J418F1	Biolegend	Cat#: 359118 / RRID:AB_2563577
CCR6 (CD196) / BV711 / G034E3	Biolegend	Cat#: 353436 / RRID:AB_2629608
CCR7 (CD197) / APC / G043H7	Biolegend	Cat#: 353213 / RRID:AB_10915474
CD11c / BUV737 / B-ly6	BD Biosciences	Cat#: 741827 / RRID:AB_2871162
CD11c / PE-Cy7 / Bu15	Biolegend	Cat#: 337216 / RRID:AB_2129790
CD123 / BV711 / 6H6	Biolegend	Cat#: 306030 / RRID:AB_2566354
CD123 / PE-Cy7 / 6H6	Biolegend	Cat#: 306010 / RRID:AB_493576
CD123 / BV711 / 9F5	Biolegend	Cat#: 563161 / RRID:AB_2738038
CD127 / PE/Dazzle 594 / A019D5	Biolegend	Cat#: 351336 / RRID:AB_2563637
CD14 / BV510 / M5E2	Biolegend	Cat#: 301841 / RRID:AB_2561379
CD14 / PE-Cy7 / M5E2	Biolegend	Cat#: 301814 / RRID:AB_389353
CD14 / APC-Cy7 / HCD14	Biolegend	Cat#: 325620 / RRID:AB_830693
CD14 / PerCP-Cy5.5 / HCD14	Biolegend	Cat#: 325622 / RRID:AB_893250
CD141 / PE / M80	Biolegend	Cat#: 344104 / RRID:AB_2255842
CD16 / BV785 / 3G8	Biolegend	Cat#: 302045 / RRID:AB_2561367
CD16 / PE-Cy7 / 3G8	BD Biosciences	Cat#: 302016 / RRID:AB_314216
CD16 / BUV737 / 3G8	BD Biosciences	Cat#: 612786 / RRID:AB_2833077
CD161 / BV421 / HP-3G10	Biolegend	Cat#: 339914 / RRID:AB_2561421
CD19 / PE/Dazzle 594 / HIB19	Biolegend	Cat#: 302252 / RRID:AB_2563560
CD19 / PE-Cy7 / SJ25C1	Biolegend	Cat#: 363012 / RRID:AB_2564203
CD19 / BUV395 / SJ25C1	Biolegend	Cat#: 563549 / RRID:AB_2738272
CD19 / FITC / HIB19	Biolegend	Cat#: 302206 / RRID:AB_314236
CD1a / PE-Cy7 / HI149	Biolegend	Cat#: 300122 / RRID:AB_2073128
CD1c / APC / L161	Biolegend	Cat#: 331524 / RRID:AB_10719956
CD24 / BUV737 / ML5	Biolegend	Cat#: 741831 / RRID:AB_2871166
CD25 / BUV737 / 2A3	Biolegend	Cat#: 612807 / RRID:AB_2916878
CD27 / FITC / O323	BD Biosciences	Cat#: 302806 / RRID:AB_314298
CD27 / APC / O323	Biolegend	Cat#: 302810 / RRID:AB_314302
CD3 / AF700 / SK7	Biolegend	Cat#: 300424 / RRID:AB_493741
CD3 / BUV737 / UCHT1	Biolegend	Cat#: 564307 / RRID:AB_2744390
CD3 / BUV395 / UCHT1	BD Biosciences	Cat#: 563546 / RRID:AB_2744387
CD34 / PE-Cy7 / 581	Biolegend	Cat#: 343516 / RRID:AB_1877251
CD38 / PE-Cy7 / HIT2	Biolegend	Cat#: 303516 / RRID:AB_2072782
CD4 / BV785 / RPA-T4	Biolegend	Cat#: 300554 / RRID:AB_2564382
CD4 / PerCP-Cy5.5 / RPA-T4	Biolegend	Cat#: 300530 / RRID:AB_893322
CD4 / APC / RPA-T4	Biolegend	Cat#: 300514 / RRID:AB_314082
CD40 / BV421 / 5C3	BD Biosciences	Cat#: 334332 / RRID:AB_2564211
CD40L (CD154) / PE / 24-31	Biolegend	Cat#: 310805 / RRID:AB_314828
CD45 / BUV395 / HI30	Biolegend	Cat#: 563792 / RRID:AB_2869519
CD45RA / PerCP-Cy5.5 / HI100	Biolegend	Cat#: 304122 / RRID:AB_893357
CD56 / PE-Cy7 / 5.1H11	Biolegend	Cat#: 362510 / RRID:AB_2563927
CD56 / BUV395 / NCAM16.2	Biolegend	Cat#: 563554 / RRID:AB_2687886
CD56 / BV421 / HCD56	Biolegend	Cat#: 318328 / RRID:AB_11218798
CD8 / BV711 / SK1	Biolegend	Cat#: 344734 / RRID:AB_2565243
CD8 / BUV737 / SK1	Biolegend	Cat#: 612754 / RRID:AB_2870085
CD80 / BV605 / 2D10	Biolegend	Cat#: 305225 / RRID:AB_11123909
CD86 / FITC / BU63	BD Biosciences	Cat#: 374204 / RRID:AB_2721574
CD95 / BV510 / DX2	Biolegend	Cat#: 305640 / RRID:AB_2629738
c-kit (CD117) / BV510 / 104D2	Biolegend	Cat#: 313220 / RRID:AB_2563804
CRTH2 (CD294) / FITC / BM16	Biolegend	Cat#: 350108 / RRID:AB_11204086
CX3CR1 / BV605 / 2A9-1	BD Biosciences	Cat#: 744488 / RRID:AB_2742268
CXCL10 / PE / 4NY8UN	Biolegend	Cat#: 12-9744-42 / RRID:AB_2572728
CXCR3 (CD183) / PE / 1C6/CXCR3	BD Biosciences	Cat#: 353705 / RRID:AB_10959652
CXCR5 (CD185) / BV711 / J252D4	BD Biosciences	Cat#: 356934 / RRID:AB_2629526
FoxP3 / PE / 206D	BD Biosciences	Cat#: 320108 / RRID:AB_492986
HLA-DR / PerCP-Cy5.5 / L243	Biolegend	Cat#: 307630 / RRID:AB_893567
HLA-DR / BV605 / L243	BD Biosciences	Cat#: 307640 / RRID:AB_2561913
IFNα2b / BV421 / 7N4-1	PBL Assay Science	Cat#: 561382 / RRID:AB_10716058
IFNβ / FITC / MMHB-3	Biolegend	Cat#: PBL-21400-3 / RRID:AB_387831
IgD / BV510 / IA6-2	BD Biosciences	Cat#: 348220 / RRID:AB_2561945
IgG / PerCP-Cy5.5 / M1310G05	Biolegend	Cat#: 410709 / RRID:AB_2565787
IgM / BV605 / MHM-88	Biolegend	Cat#: 314523 / RRID:AB_2562373
IL-12 / APC / C11.5	BD Biosciences	Cat#: 501818 / RRID:AB_2124518
IL-6 / PE-CF594 / MQ2-13A5	Biolegend	Cat#: 563543 / RRID:AB_2714015
KLRG1 / PE-Cy7 / 14C2A07	BD Biosciences	Cat#: 368614 / RRID:AB_2728371
PD-1 (CD279) / FITC / NAT105	BD Biosciences	Cat#: 367412 / RRID:AB_2572163
TCRγδ / BV786 / 11F2	BD Biosciences	Cat#: 744743 / RRID:AB_2742451
TNFα / PerCP-Cy5.5 / MAb11	Biolegend	Cat#: 502926 / RRID:AB_2204081
Vα7.2 / APC / 3C10	Biolegend	Cat#: 351708 / RRID:AB_10933246
Vδ2 / APC / B6	Biolegend	Cat#: 331418 / RRID:AB_2687324
Biological samples
Human peripheral blood, trans men cohort	amedes Medizinisches Versorgungszentrum Hamburg	N/A
Human peripheral blood, control cohort	University Medical Center Hamburg-Eppendorf	N/A
Chemicals, peptides, and recombinant proteins
Ack Lysing Buffer	Lonza Bioscience	Cat#: BP10-548E
Brefeldin A	Sigma Aldrich	Cat#: B7651-5MG
CL097	InvivoGen	Cat#: tlrl-c97
Dimethyl sulfoxide (DMSO)	Sigma Aldrich	Cat#: D85897
Fetal Bovine Serum	Sigma Aldrich	Cat#: S0615
Lymphocyte Separation Media	Capricorn	Cat#: LSM-A
Medium A (Fixation Medium)	Life Technologies	Cat#: GAS001S100
Medium B (Permeabilization Medium)	Life Technologies	Cat#: GAS002S100
Trizol	Life Technologies	Cat#: 15596018
Critical commercial assays
Chromium Next GEM Single Cell 5' Kit v2, 4 rxns	10x Genomics	Cat#: PN-1000265
Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, Plasma, TCM)	PBL Assay Science	Cat#: 41115-1
Human IFN Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)	PBL Assay Science	Cat#: 41415-1
qScriber™ cDNA Synthesis Kit	highQU GmbH	Cat#: RTK0101
Oligonucleotides
See Table S5 for a comprehensive list of primers.
Deposited data
scRNA seq data	This paper	GEO: GSE231370
Code used for analysis of scRNA seq data	This paper	https://github.com/malteborggrewe/IFN_pDC_transmencohort
Software and algorithms
AUCell v. 1.16.0	Aibar et al.54	https://scenic.aertslab.org/
Cellranger v. 7.0.0	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
enrichR v. 3.0	Kuleshov et al.56	https://github.com/wjawaid/enrichR
FlowJo v. 10.8.1	BD Biosciences	https://www.flowjo.com/
GraphPad Prism v. 9.4.0	GraphPad Software	https://www.graphpad.com/
MAST algorithm v. 1.20.0	Finak et al.53	https://github.com/RGLab/MAST
R v. 4.2.2	The R Foundation	https://www.r-project.org/
Rtsne v. 0.15	Krijthe et al.55	https://github.com/jkrijthe/Rtsne
Seurat v. 4.0.4	Hao et al.52	https://satijalab.org/seurat/