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. 2023 Sep 28;299(11):105300. doi: 10.1016/j.jbc.2023.105300

Figure 4.

Figure 4

USP11 interactions with product ubiquitin and impact of mutations.A, structure of USP11 in complex with the Ub-GGG substrate with residues subjected to mutagenesis to probe paralog-specific differences shown in magenta stick representation and a zoom in view below highlighting putative H-bonding interactions. B, thermodynamic parameters from ITC binding assays for USP11-D1D2 or indicated USP11-D1D2 mutants with mono-ubiquitin (product) or Ub-GGG, in graphical (error = SD) and table (n = 3–4 independent experiments; error = SEM) format. USP, ubiquitin-specific protease; Ub-GGG, ubiquitin-triple-gly.