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. 2023 Sep 28;299(11):105300. doi: 10.1016/j.jbc.2023.105300

Figure 6.

Figure 6

Location of “gatekeeper residue” position in blocking loop 2 and impact of mutation on linear diubiquitin cleavage in USP15.A, closeup view of the USP11 Asp886 BL2 region (in blue) in a superposition with USPs that are known to readily cleave linear diubiquitin, namely CYLD (PDB ID: 2vhf, (62)), USP2 (PDB ID: 2hd5, (30)), and USP15 (PDB ID: 6gh9, (29)). B, densitometric analyses of linear di-Ub chain cleavage assays (error = SEM; n = 3) comparing USP15-D1D2 versus USP15-D1D2 G860D. USP, ubiquitin-specific protease.