Table 1.
HDX data summary.
| Data Set | GRP94 | GRP94-ADP | GRP94-AMPNP |
|---|---|---|---|
| GRP94 | 1 μM | 1 μM | 1 μM |
| Ligand(ADP/AMPNP) | 0 μM | 20 μM | 20 μM |
| HDX reaction details | 50 mM Tris, pH 8.0, 150 mM NaCl, 10°C | ||
| HDX time course | 0, 10, 300, and 900 s | ||
| HDX control samples | non-deuterated and fully deuterated controls (GRP94) | ||
| Back-exchange (mean/IQR) | 39.51%/19.81% | ||
| # of peptides | 405 | ||
| Sequence coverage | 94% | ||
| Average peptide length/redundancy | 18.10/8.00 | ||
| Replicates (biological or technical) | 3 (biological) | ||
| Repeatability | 0.027 (average SD) | 0.074 (average SD) | 0.056 (average SD) |
| Significant differences in HDX (ΔHDX > X D) | 95% CI | ||