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360° views of MERCs larger than the 500 voxels in a COS-7 cell overexpressing the OMM–ER linker (Fig. 9), transfected with ERmoxGFP, labeled for anti-TOM-20, and imaged using 3D STED (Fig. 9). Video was rendered in Imaris 10.0 software using the contacts channel outputted from MCS-DETECT (50 frames/s). MERCs are color-coded for increasing size from 500 (blue) to 5,613 voxels (red).
