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. 2023 Jun 27;123(12):2755–2770. doi: 10.1007/s00421-023-05256-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Intermittent test protocols used during test day 2 (P2) and test day 3 (P3) where the numbers reflect the duration in minutes of each period of constant power. The constant power levels are shown to the left where PR is passive rest (no pedaling), AR is active rest, and L1, L2, etc., are individual ergometer power levels (see Table 1) intentionally designed to activate the subjects’ different metabolic energy supply systems to varying degrees